Our Sponsors
Geneious, Thomas Scientific, Integrated DNA, New England Bio, Aether Bio, and Dean of Students and Dean of Engineering at UCSC
Contact Us
1156 High St, CA 95060, US
Team Member Attributions
David Bernick
PI
Tasks
Conceptualization, Fundraising, Software, Safety, Other
In-Depth Analysis
(1) Supervision - overseeing all tasks and working being done by team, gave advice on experiments; (2) Conceptualization - helped develop initial project idea, suggested different methods to accelerate cyanobacteria growth; (3) Fundraising - pitched project to sponsors and supported all project work with rolling iGEM funding account; (4) Software - developed Stealth program and codon op, aided in the development of BLACKBIRD; (5) Safety - ensured compliance to safety guidances of the institution and respect of the policies outlined by iGEM and by the local community.
Kayla Kennamore
Student (Intern)
Tasks
Investigation, Analysis, Data Curation, Other
In-Depth Analysis
(1) Investigation - Assisted in identifying well-conserved genes for RBS and analyzed sequences using Improbizer; utilized Addgene to confirm cut sites for Eco47II and AvaII. (2) Analysis - Researched and helped identify PAMS using CRISPR-Cas finder in Limnospira fusiformis, locating three relevant CRISPR arrays and confirming potential PAMs. (3) Data Curation - Compiled and organized findings on PAMs, TAG sequences, and RBS for documentation. Documented sequence variations using STEALTH identifying a variant for Eco47II (4) Social Media Management - Operated the team’s social media account to enhance outreach and communication efforts
Neely Pfeiffer
Student (Intern)
Tasks
Background Research, Investigation, Public Engagement, Wiki Coding, Visualization
In-Depth Analysis
(1) Background Research- Self recognition mechanisms such as PAM and TAG for Cas 9 and Cas 10 systems, Golden Gate Assembly, Cas 12a, Lambda-Red Recombineering, General Prokaryotic Argonaute Systems (2) Investigation- Found the sequences for methylation targeted by the pRL528 plasmid within Limnospira and UTEX 2973 involving Eco47II and AvaII, Identified/Confirmed the lack of PAM sequence within Limnospira Fusiformis SAG 85 (3) Public Engagement- Guide for Soquel High student meetup/ lab rotations (4) Wiki Coding- Created informative youtube videos for team uniformity with image insertion and debugging, created navigation system/hamburger (5) Visualization- Markup for wiki layout with focus on homepage, main navigational menu, and scroll bar/ progress bar.
External Contributions
Robin Rounthwaite
Advisor
Tasks
Software, Wiki Development, Other
In-Depth Analysis
(1) Supervision - overseeing all tasks and working being done by team, gave advice on software; (2) Software - assisted in the coding aspect of the project; (3) Wiki Development - assisted team members to set up proper GitLab working structure, as well as helped troubleshoot multiple wiki complications
Julia Saltz
Advisor
Tasks
Background Research, Protocol Troubleshooting, Other
In-Depth Analysis
(1) Supervision - overseeing all tasks and working being done by team; (2) Background Research - read papers and discussed contents with the team; (3) Protocol Troubleshooting - assisted with Miniprep, MagBead-related Troubleshooting
UCSC Jack Baskin Engineering Dean
Institution Type: Academic
Relationship to Team: Sponsor
Tasks
Fundraising
Specific Task
Provided financial assistance
UCSC Physical and Biological Science Dean
Institution Type: Academic
Relationship to Team: Sponsor
Tasks
Fundraising
Specific Task
Provided financial assistance
UCSC Stevenson College
Institution Type: Academic
Relationship to Team: Sponsor
Tasks
Fundraising
Specific Task
Provided financial assistance
Santa Cruz Foundation
Institution Type: Nonprofit
Relationship to Team: Sponsor
Tasks
Fundraising
Specific Task
Provided financial assistance
University of California, Berkeley
Institution Type: Academic
Relationship to Team: Contractor
Tasks
Investigation
Specific Task
Performed Sanger sequencing on plasmid Golden Gate Assembly samples
Alistair McCormick
Institution Type: Academic
Relationship to Team: External Advisor
Tasks
Conceptualization, Background Research
Specific Task
(1)Conceptualization- gave us advice on culturing cyanobacteria; (2)Project Administration- helped us understand the conjugation techniques, introduced us to the fast-growing strain PCC11901
Ravendran Vasudevan
Institution Type: Academic
Relationship to Team: External Advisor
Tasks
Conceptualization, Project Administration
Specific Task
(1)Conceptualization- gave us advice on using the MoClo kits; (2)Project Administration- helped us understand the MoClo systems
Shan Huang
Institution Type: Academic
Relationship to Team: External Advisor
Tasks
Conceptualization, Project Administration
Specific Task
(1)Conceptualization- talked to us about his understnading of argonautes (2) Project Administration- helped up plan out our Argonaute Workflow
Max Schubert
Institution Type: Academic
Relationship to Team: External Advisor
Tasks
Investigation, Conceptualization
Specific Task
(1)Conceptualization- Gave us advice on cyanobacteria culturing; (2)Investigation- gave us our working strains UTEX 3154, 3086, 3222
Susan Golden
Institution Type: Academic
Relationship to Team: External Advisor
Tasks
Conceptualization
Specific Task
(1) Conceptualization- gave us advice on the culturing media for cyanobacteria
Hannah Tabakh
Institution Type: Industry
Relationship to Team: External Advisor
Tasks
Conceptualization
Specific Task
(1) Conceptualization- gave us advice on STAR protocol (a Lumen Protocol for culturing Limnospira Platensis
Ben Jester
Institution Type: Industry
Relationship to Team: External Advisor
Tasks
Conceptualization
Specific Task
(1) Conceptualization- gave us advice on STAR protocol (a Lumen Protocol for culturing Limnospira Platensis
Lauren Goetsch
Institution Type: Industry
Relationship to Team: External Advisor
Tasks
Conceptualization
Specific Task
(1) Conceptualization- helped us learn more about handling the strain and gain insight within our project
Damian Carrieri
Institution Type: Industry
Relationship to Team: External Advisor
Tasks
Conceptualization, Project Administration
Specific Task
(1) Conceptualization- talked to us about his experience on transforming cyanobacteria (2)Project Administration- helped us understand the importance of selectable markers
Olivia Meyer
Institution Type: Industry
Relationship to Team: External Advisor
Tasks
Public Engagement
Specific Task
(1) Public Engagement- helped us understand the community perspective on our project
Lydia Mapstone
Institution Type: Academic
Relationship to Team: External Advisor
Tasks
Conceptualization, Public Engagement
Specific Task
(1) Public Engagement- helped us understand the community perspective on our project (2)Conceptualization- gave us initial idea of what a cyanobacteria workflow would look like
Nehal Pfeiffer
Institution Type: Other
Relationship to Team: External Advisor
Tasks
Public Engagement
Specific Task
(1) Public Engagement- coordinated with us to set up for the Soquel HS Biology class come into lab
Timeline
Recruit Team Members
Start Date
June 26th, 2023
End Date
December 7th, 2023
Total Work Time
23 Weeks
Development of Project Plan
Start Date
May 24th, 2024
End Date
September 20th, 2024
Total Work Time
17 Weeks
Dry Laboratory Activities
Start Date
June 24th, 2024
End Date
September 20th, 2024
Total Work Time
13 Weeks
Wet Laboratory Activities
Start Date
July 1st, 2024
End Date
October 28th, 2024
Total Work Time
17 Weeks
Building the Team Wiki
Start Date
August 21st, 2024
End Date
October 2nd, 2024
Total Work Time
6 Weeks